Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
Charlotte Chambrion
(1)
,
Mallorie Depond
(1)
,
Lucia Angella
(1)
,
Oussama Mouri
(2)
,
Eric Kendjo
(2)
,
Aurélie Fricot-Monsinjon
(1)
,
Camille Roussel
(1, 3)
,
Sylvestre Biligui
(2)
,
Ilhame Tantaoui
(2)
,
Aida Taieb
(2)
,
Nicolas Argy
(4)
,
Sandrine Houzé
(4)
,
Renaud Piarroux
(2)
,
Jean-Yves Siriez
(5)
,
Stéphane Jaureguiberry
(6)
,
Sébastien Larréché
(7)
,
Marc Théllier
(2)
,
Nicolas Cenac
(8)
,
Pierre Buffet
(1)
,
Papa Alioune Ndour
(1)
1
BIGR (UMR_S_1134 / U1134) -
Biologie Intégrée du Globule Rouge
2 CHU Pitié-Salpêtrière [AP-HP]
3 Service d'immuno-hématologie pédiatrique [CHU Necker]
4 AP-HP - Hôpital Bichat - Claude Bernard [Paris]
5 Hôpital Robert Debré Paris
6 Hôpital Bicêtre [AP-HP, Le Kremlin-Bicêtre]
7 HIA Begin - Hôpital d'Instruction des Armées Begin [Saint-Mandé, France]
8 IRSD - Institut de Recherche en Santé Digestive
2 CHU Pitié-Salpêtrière [AP-HP]
3 Service d'immuno-hématologie pédiatrique [CHU Necker]
4 AP-HP - Hôpital Bichat - Claude Bernard [Paris]
5 Hôpital Robert Debré Paris
6 Hôpital Bicêtre [AP-HP, Le Kremlin-Bicêtre]
7 HIA Begin - Hôpital d'Instruction des Armées Begin [Saint-Mandé, France]
8 IRSD - Institut de Recherche en Santé Digestive
Nicolas Argy
- Fonction : Auteur
- PersonId : 782310
- ORCID : 0000-0002-0458-2664
- IdRef : 155847104
Papa Alioune Ndour
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 1134802
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Résumé
In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls ( p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls ( p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected.
Domaines
Médecine humaine et pathologie Physiologie [q-bio.TO] Organisation et fonctions cellulaires [q-bio.SC]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
|
Résumé |
en
In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls ( p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls ( p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected.
|
Auteur(s) |
Charlotte Chambrion
1
, Mallorie Depond
1
, Lucia Angella
1
, Oussama Mouri
2
, Eric Kendjo
2
, Aurélie Fricot-Monsinjon
1
, Camille Roussel
1, 3
, Sylvestre Biligui
2
, Ilhame Tantaoui
2
, Aida Taieb
2
, Nicolas Argy
4
, Sandrine Houzé
4
, Renaud Piarroux
2
, Jean-Yves Siriez
5
, Stéphane Jaureguiberry
6
, Sébastien Larréché
7
, Marc Théllier
2
, Nicolas Cenac
8
, Pierre Buffet
1
, Papa Alioune Ndour
1
1
BIGR (UMR_S_1134 / U1134) -
Biologie Intégrée du Globule Rouge
( 1005032 )
- 6 rue Alexandre Cabanel - 75739 Paris cedex 15
- France
2
CHU Pitié-Salpêtrière [AP-HP]
( 353778 )
- 47-83 Boulevard de l'Hôpital, 75013 Paris
- France
3
Service d'immuno-hématologie pédiatrique [CHU Necker]
( 19365 )
- 149 Rue de Sèvres, 75015 Paris
- France
4
AP-HP - Hôpital Bichat - Claude Bernard [Paris]
( 300156 )
- 46 Rue Henri Huchard, 75018 Paris
- France
5
Hôpital Robert Debré Paris
( 116327 )
- Hôpital Robert Debré, service ORL, 48 Boulevard Sérurier, 75019 Paris
- France
6
Hôpital Bicêtre [AP-HP, Le Kremlin-Bicêtre]
( 312432 )
- 78 Rue du Général Leclerc 94270 Le Kremlin-Bicêtre
- France
7
HIA Begin -
Hôpital d'Instruction des Armées Begin [Saint-Mandé, France]
( 37970 )
- 69 avenue de Paris, 94160 Saint-Mandé
- France
8
IRSD -
Institut de Recherche en Santé Digestive
( 1002454 )
- CHU PURPAN, Place du Docteur Baylac
CS 60039, 31024 TOULOUSE CEDEX 3
- France
|
Licence |
Paternité
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2022-04-05
|
Volume |
13
|
Page/Identifiant |
8 p.
|
Version du document |
version éditeur
|
Public visé |
Scientifique
|
Numéro d'article |
|
Sous-type de document pour les Articles |
Research article
|
Domaine(s) |
|
Collaboration/Projet |
|
Projet(s) ANR |
|
Financement |
|
Mots-clés |
en
P, falcipaprum, artemisinin derivatives, malaria anemia, RBC deformability, spleen filtering funcion, pitted or once infected RBC, membrane lipid balance
|
DOI | 10.3389/fphys.2022.875189 |
Pubmed Id | 35480048 |
PubMed Central | PMC9037233 |
UT key WOS | 000792456400001 |
Origine :
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