Genipap (Genipa americana L.) is an exotic fruit largely consumed and well known, in Amazonian pharmacopeia, to treat anemia, measles and uterine cancer. It is also used as a diuretic, digestive, healing, laxative and antiseptic. The aim of this study was to apply an untargeted metabolomics strategy to determine biomarkers of food intake after short-term consumption of genipap juice. Sixteen healthy adult men were administered jenipap juice (250 mL) twice a day for three weeks. Before and after the three weeks of consumption. the subjects drank a control drink, and they consumed a standard diet. Urine was collected after 0-6 h, 6-12 h and 12-24 h. An ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS)-based metabolomics approach was applied to analyze the urine samples. Principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to highlight experimental differences between groups. The value of the area under the curve (AUC) of the receiver operator characteristic (ROC) curve validated the identified biomarkers. Thirty-one statistically affected urinary metabolites were putatively identified and were mainly related to iridoids family, medium-chain fatty acids, and polyphenols. Also a group of urinary markers including dihydrocaffeic acid (DHCA), 1-(4-hydroxyphenyl)-1,2-propanediol and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid were established as biomarkers of genipap consumption. Our findings have established a comprehensive panel of changes in the urinary metabolome and provided information to monitor endogenous alterations that are linked to genipap juice intake. These data should be used in further studies to understand the health implications of genipap juice consumption.
Genipap (Genipa americana L.) juice intake biomarkers after medium-term consumption
Résumé
en
Genipap (Genipa americana L.) is an exotic fruit largely consumed and well known, in Amazonian pharmacopeia, to treat anemia, measles and uterine cancer. It is also used as a diuretic, digestive, healing, laxative and antiseptic. The aim of this study was to apply an untargeted metabolomics strategy to determine biomarkers of food intake after short-term consumption of genipap juice. Sixteen healthy adult men were administered jenipap juice (250 mL) twice a day for three weeks. Before and after the three weeks of consumption. the subjects drank a control drink, and they consumed a standard diet. Urine was collected after 0-6 h, 6-12 h and 12-24 h. An ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS)-based metabolomics approach was applied to analyze the urine samples. Principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to highlight experimental differences between groups. The value of the area under the curve (AUC) of the receiver operator characteristic (ROC) curve validated the identified biomarkers. Thirty-one statistically affected urinary metabolites were putatively identified and were mainly related to iridoids family, medium-chain fatty acids, and polyphenols. Also a group of urinary markers including dihydrocaffeic acid (DHCA), 1-(4-hydroxyphenyl)-1,2-propanediol and 3-carboxy-4-methyl-5-propyl-2-furanpropionic acid were established as biomarkers of genipap consumption. Our findings have established a comprehensive panel of changes in the urinary metabolome and provided information to monitor endogenous alterations that are linked to genipap juice intake. These data should be used in further studies to understand the health implications of genipap juice consumption.
1
UFPA -
Federal University of Para - Universidade Federal do Pará - UFPA [Belém, Brazil]
( 441981 )
- Rua Itaipu, 36 - Vila Permanente, Tucuruí - PA, 68464-000, Belem - Brésil
- Brésil
2
Naturex SA [Avignon]
( 1043935 )
- Avignon
- France
3
UMR Qualisud -
Démarche intégrée pour l'obtention d'aliments de qualité
( 31878 )
- TA B95/16 - 73 rue Jean-François Breton 34398 Montpellier Cedex 5, France
- France
Centre de Coopération Internationale en Recherche Agronomique pour le Développement UMR95 ( 11574 )
;
Centre international d'études supérieures en sciences agronomiques ( 92699 )
;
Avignon Université ( 195507 )
;
Université de La Réunion ( 300316 )
;
Université de Montpellier ( 410122 )
;
Institut national d’études supérieures agronomiques de Montpellier ( 474617 )
4
C2VN -
Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research
( 1002339 )
- Faculté de Médecine de la Timone 27 boulevard Jean Moulin 13385 MARSEILLE CEDEX 5
- France
Aix Marseille Université UMR_S1263 ( 198056 )
;
Institut National de la Santé et de la Recherche Médicale UMR_S1263 ( 303623 )
;
Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement UMR1260 ( 577435 )
5
Cirad-PERSYST -
Département Performances des systèmes de production et de transformation tropicaux
( 94474 )
- Avenue Agropolis TA B-DIR / 09 34398 Montpellier Cedex 5 France
- France
Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( 11574 )
Langue du document
Anglais
Nom de la revue
Food Research International
(ISSN : 0963-9969)
Publié par Elsevier
Revue non référencée dans Sherpa-Romeo
Vulgarisation
Non
Comité de lecture
Oui
Audience
Internationale
Date de publication
2020-11
Volume
137
Page/Identifiant
109375
Public visé
Scientifique
Domaine(s)
Sciences du Vivant [q-bio]/Alimentation et Nutrition
Financement
French company Naturex [CIFRE] 2015/0001
Mots-clés
en
Untargeted metabolomics, Urine, LC-MS, Food biomarkers intake, Iridoids, Exotic fruit
Livia Dickson, Mathieu Tenon, Ljubica Svilar, Pascale Fanca-Berthon, Jean-Charles Martin, et al.. Genipap (Genipa americana L.) juice intake biomarkers after medium-term consumption. Food Research International, 2020, 137, pp.109375. ⟨10.1016/j.foodres.2020.109375⟩. ⟨hal-03155448⟩