Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon
Serges Tchatchouang
(1, 2, 3)
,
Ariane Nzouankeu
(1)
,
Eva Hong
(2)
,
Aude Terrade
(2)
,
Mélanie Denizon
(2)
,
Ala-Eddine Deghmane
(2)
,
Suzie Moyo Tetang Ndiang
(4)
,
Eric-Walter Pefura-Yone
(5)
,
Véronique Penlap Beng
(3)
,
Richard Njouom
(1)
,
Marie-Christine Fonkoua
(1)
,
Muhamed-Kheir Taha
(2)
Ala-Eddine Deghmane
- Fonction : Auteur
- PersonId : 746652
- IdHAL : ala-eddinedeghmane
- ORCID : 0000-0003-2887-1732
Muhamed-Kheir Taha
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 173896
- IdHAL : muhamed-kheir-taha
- ORCID : 0000-0002-0716-3174
- IdRef : 050218034
Connectez-vous pour contacter l'auteur
Résumé
Objectives: To identifyHaemophilus species and characterize antimicrobial susceptibility of isolates from patients with respiratory tract infections (RTIs) in Cameroon.
Methods: Isolates (n = 95) were from patients with RTIs obtained from two Hospitals in Yaoundé, Cameroon. Isolates were identified by biochemical assay, PCR-based method, MALDI-TOF and whole genome sequencing. Antibiotic minimum inhibitory concentrations were determined by E-test.
Results: H. influenzae was the most prevalent species varying from 76.8% to 84.2% according to different methods. The isolates were mainly nontypable (n = 70, 96%). Three isolates of H. influenzae were capsulated (b, e and f). The isolates were genetically diverse and 40 unique sequence types were identified including 11 new ones. Resistance to ampicillin was observed among 55.3% (52/94) and 9% (14/52) produced TEM-1 β-lactamase. PBP3 mutations occurred in 57.7% of ampicillin resistant isolates (30/52). Eleven isolates were chloramphenicol resistant with 80% producing chloramphenicol acetyltransferase (8/10). Four Haemophilus isolates were rifampicin resistant with two mutations in rpoB gene. Five isolates were ciprofloxacin resistant and harbored mutations in the quinolone resistance determining regions of gyrA and parC genes.
Conclusion: H. influenzae isolates are highly diverse and show high levels of antibiotic resistance. H. influenzae serotype b is still circulating in the post-vaccination era.
Domaines
BactériologieFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
Objectives: To identifyHaemophilus species and characterize antimicrobial susceptibility of isolates from patients with respiratory tract infections (RTIs) in Cameroon.
Methods: Isolates (n = 95) were from patients with RTIs obtained from two Hospitals in Yaoundé, Cameroon. Isolates were identified by biochemical assay, PCR-based method, MALDI-TOF and whole genome sequencing. Antibiotic minimum inhibitory concentrations were determined by E-test.
Results: H. influenzae was the most prevalent species varying from 76.8% to 84.2% according to different methods. The isolates were mainly nontypable (n = 70, 96%). Three isolates of H. influenzae were capsulated (b, e and f). The isolates were genetically diverse and 40 unique sequence types were identified including 11 new ones. Resistance to ampicillin was observed among 55.3% (52/94) and 9% (14/52) produced TEM-1 β-lactamase. PBP3 mutations occurred in 57.7% of ampicillin resistant isolates (30/52). Eleven isolates were chloramphenicol resistant with 80% producing chloramphenicol acetyltransferase (8/10). Four Haemophilus isolates were rifampicin resistant with two mutations in rpoB gene. Five isolates were ciprofloxacin resistant and harbored mutations in the quinolone resistance determining regions of gyrA and parC genes.
Conclusion: H. influenzae isolates are highly diverse and show high levels of antibiotic resistance. H. influenzae serotype b is still circulating in the post-vaccination era.
|
Titre |
en
Analysis of Haemophilus species in patients with respiratory tract infections in Yaoundé, Cameroon
|
Auteur(s) |
Serges Tchatchouang
1, 2, 3
, Ariane Nzouankeu
1
, Eva Hong
2
, Aude Terrade
2
, Mélanie Denizon
2
, Ala-Eddine Deghmane
2
, Suzie Moyo Tetang Ndiang
4
, Eric-Walter Pefura-Yone
5
, Véronique Penlap Beng
3
, Richard Njouom
1
, Marie-Christine Fonkoua
1
, Muhamed-Kheir Taha
2
1
Centre Pasteur du Cameroun
( 55917 )
- B.P. 1274 Yaoundé
- Cameroun
2
CNR -
Centre National de Référence des Méningocoques et Haemophilus influenzae - National Reference Center Meningococci and Haemophilus influenzae
( 169666 )
- Unité Infections bactériennes invasives
25-28 rue du Docteur Roux - 75724 Paris Cedex 15
- France
3
UY1 -
Université de Yaoundé I
( 217395 )
- B.P. 337 Yaoundé
- Cameroun
4
Centre Hospitalier Essos [Yaoundé, Cameroun]
( 129092 )
- Yaoundé
- Cameroun
5
Jamot hospital
( 1071655 )
- P.O. Box 4021, Yaoundé, Cameroon
- Cameroun
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2020-11
|
Volume |
100
|
Page/Identifiant |
12-20
|
Licence |
Paternité - Pas d'utilisation commerciale - Pas de modification
|
Mots-clés (Mesh) |
|
Financement |
|
Domaine(s) |
|
Mots-clés |
en
Haemophilus species, Respiratory tract infection, antibiotic resistance, typing, whole genome sequencing
|
DOI | 10.1016/j.ijid.2020.08.040 |
Pubmed Id | 32827751 |
Origine :
Publication financée par une institution
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