Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
Franck-Martin Obam Mekanda
(1, 2)
,
Chavely Gwladys Monamele
(1)
,
Frédy Brice Simo Nemg
(1)
,
Gilde Martial Yonga
(1)
,
Diane Ouapi
(1)
,
Véronique Penlap Beng
(2)
,
Christophe Batejat
(3, 4)
,
Valerie Caro
(3, 4)
,
Jean-Claude Manuguerra
(4, 3)
,
Maurice Demanou
(1)
Valerie Caro
- Fonction : Auteur
- PersonId : 747321
- IdHAL : valerie-caro
- ORCID : 0000-0001-8477-5708
Jean-Claude Manuguerra
- Fonction : Auteur
- PersonId : 920746
- IdHAL : jean-claude-manuguerra
- ORCID : 0000-0002-5202-6531
- IdRef : 050363506
Maurice Demanou
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 884164
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Résumé
The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination.
Domaines
Maladies infectieusesFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination.
|
Titre |
en
Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
|
Auteur(s) |
Franck-Martin Obam Mekanda
1, 2
, Chavely Gwladys Monamele
1
, Frédy Brice Simo Nemg
1
, Gilde Martial Yonga
1
, Diane Ouapi
1
, Véronique Penlap Beng
2
, Christophe Batejat
3, 4
, Valerie Caro
3, 4
, Jean-Claude Manuguerra
4, 3
, Maurice Demanou
1
1
Centre Pasteur du Cameroun
( 55917 )
- B.P. 1274 Yaoundé
- Cameroun
2
UY1 -
Université de Yaoundé I
( 217395 )
- B.P. 337 Yaoundé
- Cameroun
3
CIBU -
Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
( 31437 )
- Département Infection et Epidémiologie, 25-28 rue du Docteur Roux F-75724 Paris Cedex 15
- France
4
ERI -
Environnement et Risques infectieux - Environment and Infectious Risks
( 237998 )
- Département Infection et Epidémiologie, 28, rue du Docteur Roux, 75724 Paris Cedex 15
- France
|
Licence |
Paternité
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2019-09-25
|
Volume |
14
|
Numéro |
9
|
Page/Identifiant |
e0222428
|
Mots-clés (Mesh) |
|
Financement |
|
Domaine(s) |
|
DOI | 10.1371/journal.pone.0222428 |
Pubmed Id | 31553766 |
PubMed Central | PMC6760898 |
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