Skip to Main content Skip to Navigation
Journal articles

Alternative Splicing of TAF6: Downstream Transcriptome Impacts and Upstream RNA Splice Control Elements

Abstract : The TAF6δ pathway of apoptosis can dictate life versus death decisions independently of the status of p53 tumor suppressor. TAF6δ is an inducible pro-apoptotic subunit of the general RNA polymerase II (Pol II) transcription factor TFIID. Alternative splice site choice of TAF6δ has been shown to be a pivotal event in triggering death via the TAF6δ pathway, yet nothing is currently known about the mechanisms that promote TAF6δ splicing. Furthermore the transcriptome impact of the gain of function of TAF6δ versus the loss of function of the major TAF6α splice form remains undefined. Here we employ comparative microarray analysis to show that TAF6δ drives a transcriptome profile distinct from that resulting from depletion of TAF6α. To define the cis-acting RNA elements responsible for TAF6δ alternative splicing we performed a mutational analysis of a TAF6 minigene system. The data point to several new RNA elements that can modulate TAF6δ and also reveal a role for RNA secondary structure in the selection of TAF6δ.
Document type :
Journal articles
Complete list of metadatas

Cited literature [54 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01365767
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Tuesday, September 13, 2016 - 3:56:42 PM
Last modification on : Tuesday, March 17, 2020 - 2:40:03 PM
Document(s) archivé(s) le : Wednesday, December 14, 2016 - 2:12:02 PM

File

10.1371-journal.pone.0102399.P...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Catherine Kamtchueng, Marie-Eve Stébenne, Aurélie Delannoy, Emmanuelle Wilhelm, Hélène Léger, et al.. Alternative Splicing of TAF6: Downstream Transcriptome Impacts and Upstream RNA Splice Control Elements. PLoS ONE, Public Library of Science, 2014, 9 (7), pp.e102399. ⟨10.1371/journal.pone.0102399⟩. ⟨hal-01365767⟩

Share

Metrics

Record views

284

Files downloads

437