Modeling the bioconversion of polysaccharides in a continuous reactor: A case study of the production of oligo-galacturonates by Dickeya dadantii

Jacques-Alexandre Sepulchre 1, 2 Sylvie Reverchon 3 Jean-Luc Gouzé 4, 2 William Nasser 3
3 CRP - Chromatine et Régulation de la Pathogénie bactérienne
MAP - Microbiologie, adaptation et pathogénie
4 BIOCORE - Biological control of artificial ecosystems
CRISAM - Inria Sophia Antipolis - Méditerranée , INRA - Institut National de la Recherche Agronomique, LOV - Laboratoire d'océanographie de Villefranche
Abstract : In the quest for a sustainable economy of the earth resources and for renewable sources of energy, a promising avenue is to exploit the vast quantity of polysaccharide molecules contained in green wastes. To that end, the decomposition of pectin appears to be an interesting target because this polymeric carbohydrate is abundant in many fruit pulps and soft vegetables. To quantitatively study this degradation process, here we designed a bioreactor that is continuously fed with de-esterified pectin (PGA). Thanks to the pectate lyases produced by bacteria cultivated in the vessel, the PGA is depolymerized into oligo-galacturonates (UGA) which is continuously extracted from the tank. A mathematical model of our system predicted that the conversion efficiency of PGA into UGA increases in a range of coefficient of dilution until reaching an upper limit where the fraction of UGA that is extracted from the bioreactor is maximized. Results from experiments with a continuous reactor hosting a strain of the plant pathogenic bacterium Dickeya dadantii and in which the dilution coefficients were varied, quantitatively validated the predictions of our model. A further theoretical analysis of the system enabled an a priori comparison of the efficiency of eight other pectate lyases-producing microorganisms with that of D. dadantii. Our findings suggest that D. dadantii is as the most efficient microorganism and therefore the best candidate for a practical implementation of our scheme for the bioproduction of UGA from PGA.
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Jacques-Alexandre Sepulchre, Sylvie Reverchon, Jean-Luc Gouzé, William Nasser. Modeling the bioconversion of polysaccharides in a continuous reactor: A case study of the production of oligo-galacturonates by Dickeya dadantii. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2019, 294 (5), pp.1753-1762. ⟨10.1074/jbc.RA118.004615⟩. ⟨hal-01952846⟩

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