Arrêt de service programmé du vendredi 10 juin 16h jusqu’au lundi 13 juin 9h. Pour en savoir plus
Accéder directement au contenu Accéder directement à la navigation
Autre publication (Article de blog)

iGEM REPORT: Gotta Detect ‘Em All: a multi-STI sensor based on aptamers

Abstract : Nowadays, STIs constitute a major public health issue. Indeed, treatments are often started too late because of belated diagnosis resulting in health problems, such as sterility. If prevention is probably the most effective action one can take to prevent the spread of STIs, early detection could help limit their deleterious effects. In this work, a new diagnosis approach based on aptamers is presented. Bound to paper, they allow the detection of HIV and Hepatitis B biomarkers from a blood sample. The associated device is composed of an anchor, the streptavidin protein, allowing the fixation of the aptamer to the paper via biotin (see graphical abstract). With this system, the HIV-1 Reverse Transcriptase (BBa_K1934060 and BBa_K1934061: protein subunits p51 and p66) and HBsAg (surface antigen of Hepatitis B) are specifically targeted. Then, the biomarker/aptamer complex is detected by two methods. The first one is based on fluorescence. As a proof of concept, a paired ATP/aptamer was used and enabled to successfully detect ATP up to 10 µmol.L-1. However, the signal was not detectable with naked eyes or with a cell phone equipped with blue and green filters either. Therefore, a lateral flow assay with nano-sized latex black beads was tested. This second technique showed that a protein biomarker, such as thrombin, could be complexed with latex beads coated with aptamers, in liquid. Finally, the ultimate step, migration of the latex beads inside paper, needs further optimization. Moreover, to easily handle several STI-tests on a single paper strip, an innovative bio-sourced PLA casing was designed and 3D printed to offer an additional intuitive user-interface.
Keywords : STI sensor aptamers
Type de document :
Autre publication (1)
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-03463368
Contributeur : Yoann Louis Connectez-vous pour contacter le contributeur
Soumis le : vendredi 7 janvier 2022 - 09:47:09
Dernière modification le : mardi 12 avril 2022 - 10:12:08
Archivage à long terme le : : vendredi 8 avril 2022 - 18:15:27

Fichier

Igem plos blog.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

  • HAL Id : hal-03463368, version 1

Citation

Julien Orlans, Paul Zanoni, Louis Becquey, Mathilde Gonthier, Mathieu Borel, et al.. iGEM REPORT: Gotta Detect ‘Em All: a multi-STI sensor based on aptamers. 2017. ⟨hal-03463368⟩

Partager

Métriques

Consultations de la notice

32

Téléchargements de fichiers

1